Nitrogen remobilization in shoots of Paris polyphylla is altered by gibberellic acid application during senescence

Nitrogen remobilization in shoots of Paris polyphylla is altered by gibberellic acid application during senescence

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Nitrogen remobilization during SectionalComponent senescence has been studied in perennial herb Paris polyphylla.We analyzed changes in N content, amino acids, N-remobilization enzymes and effects of gibberellic acid (GA) during natural senescence.There was a gradual decrease in the contents of N, chlorophyll and soluble proteins and activities of glutamine synthetase (GS; EC 6.3.

1.2) and glutamate dehydrogenase (GLDH; EC 1.4.1.

2).Activity staining and Western blots showed that GS2 activity decreased, whereas GS1 activity was relatively stable over time.In contrast, the C/N ratio and total amino acid content increased.Among individual amino acids, the proportions of glutamine (Gln) and asparagine (Asn) increased, and proportions of arginine, aspartate and glycine decreased.

Treatment with GA slowed the senescence and retarded decreases in the activities of GS and Extension Cords GLDH and the contents of N, chlorophyll and soluble proteins.Conversely, this treatment slowed increases in the C/N ratio, total free amino acid content, and proportions of Gln and Asn.We conclude that low N resorption efficiency during senescence of P.polyphylla results from a sharp decrease in N remobilization enzyme activity.